Coding

Part:BBa_K3561028:Design

Designed by: Poon Curtis Long Yiu   Group: iGEM20_HK_SSC   (2020-10-23)


TSNAVAWTLRCL-CBD


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 37
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 37
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 37
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 37
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 37
    Illegal AgeI site found at 145
    Illegal AgeI site found at 355
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We have added a NheI and a PStI cut site at the start and end of the sequence respectively. An EcoRI cut site is added in between the peptide and the CBD. The codons are also optimised.


Source

We have modified the peptide from library peptides with our own mutations. The CBD is provided Prof. Shen Long Tsai.

References